Calmodulin Is a Functional Regulator of Cav1.4 L-type Ca Channels*

نویسندگان

  • Kristina Griessmeier
  • Hartmut Cuny
  • Katrin Rötzer
  • Oliver Griesbeck
  • Hartmann Harz
  • Martin Biel
  • Christian Wahl-Schott
چکیده

Kristina Griessmeier, Hartmut Cuny, Katrin Rötzer, Oliver Griesbeck, Hartmann Harz, Martin Biel, and Christian Wahl-Schott From the Center for Integrated Protein Science (CIPS-M) and Zentrum für Pharmaforschung, Department Pharmazie, Ludwig-Maximilians-Universität München, D-81377 München, the Max Planck Institute of Neurobiology, D-82152 Martinsried, and the BioImaging Zentrum, Ludwig-Maximilians-Universität München, D-81377 München, Germany

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Calmodulin is a functional regulator of Cav1.4 L-type Ca2+ channels.

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Structural insights into activation of the retinal L-type Ca²⁺ channel (Cav1.4) by Ca²⁺-binding protein 4 (CaBP4).

CaBP4 modulates Ca(2+)-dependent activity of L-type voltage-gated Ca(2+) channels (Cav1.4) in retinal photoreceptor cells. Mg(2+) binds to the first and third EF-hands (EF1 and EF3), and Ca(2+) binds to EF1, EF3, and EF4 of CaBP4. Here we present NMR structures of CaBP4 in both Mg(2+)-bound and Ca(2+)-bound states and model the CaBP4 structural interaction with Cav1.4. CaBP4 contains an unstruc...

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The retinal L-type Ca2+ channel Cav1.4 is distinguished from all other members of the high voltage-activated (HVA) Ca2+ channel family by lacking Ca2+-calmodulin-dependent inactivation. In synaptic terminals of photoreceptors and bipolar cells, this feature is essential to translate graded membrane depolarizations into sustained Ca2+ influx and tonic glutamate release. The sequences conferring ...

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Proper function of Cav1.4 L-type calcium channels is crucial for neurotransmitter release in the retina. Our understanding about how different levels of Cav1.4 channel activity affect retinal function is still limited. In the gain-of-function mouse model Cav1.4-IT we expected a reduction in the photoreceptor dynamic range but still transmission toward retinal ganglion cells. A fraction of Cav1....

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تاریخ انتشار 2009